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zymo dna clean  (Zymo Research)
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( A ) Workflow of <t>DNA</t> O-MAP integrated with sample multiplexing quantitative proteomics. ( B ) Schematic of the three DNA loci examined in the TMT16plex experiment: pericentromeric alpha satellites, telomeres, and mitochondrial genomes. ( C ) Co-localization of DNA fluorescent in situ hybridization (FISH) and the streptavidin staining of the proteins biotinylated by DNA O-MAP targeting the pericentromeric alpha satellites, telomeres, and mitochondrial genomes. Scale bar: 5 µm. ( D ) Principal component analysis of scaled intensities of proteins enriched by the pan-alpha probe, telomere probe, mitochondrial <t>genome</t> <t>oligo</t> pool, and no-primary-probe control. ( E ) Unsupervised hierarchical clustering of scaled intensities of proteins enriched by the pan-alpha probe, telomere probe, mitochondrial genome oligo pool, and no-primary-probe control. ( F ) Log 2 fold change of proteins compared to no-primary-probe control, grouped by HPA subcellular location. Significance calculated based on Welch’s t -test for pairwise comparisons (****p-value <0.0001). Log 2 fold change of proteins compared to mitochondrial probe enriched proteins for the RNA Polymerases ( G ), mtDNA nucleoid packaging proteins ( H ), Shelterin ( I ), and CENP-A nucleosomal complexes ( J ). Significance calculated based on Welch’s t -test for pairwise comparisons (p-value: *<0.05, **<0.01, ***<0.001, ****<0.0001).
Zymo Dna Clean, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+clean+concentrator+100/DNA+Clean+%26+Concentrator-100/pmc13056364-243-7-7
Average 95 stars, based on 1 article reviews
zymo dna clean - by Bioz Stars, 2026-11
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am9914g dna clean concentrator 100 zymo research  (Zymo Research)
99
Zymo Research am9914g dna clean concentrator 100 zymo research
( A ) Workflow of <t>DNA</t> O-MAP integrated with sample multiplexing quantitative proteomics. ( B ) Schematic of the three DNA loci examined in the TMT16plex experiment: pericentromeric alpha satellites, telomeres, and mitochondrial genomes. ( C ) Co-localization of DNA fluorescent in situ hybridization (FISH) and the streptavidin staining of the proteins biotinylated by DNA O-MAP targeting the pericentromeric alpha satellites, telomeres, and mitochondrial genomes. Scale bar: 5 µm. ( D ) Principal component analysis of scaled intensities of proteins enriched by the pan-alpha probe, telomere probe, mitochondrial <t>genome</t> <t>oligo</t> pool, and no-primary-probe control. ( E ) Unsupervised hierarchical clustering of scaled intensities of proteins enriched by the pan-alpha probe, telomere probe, mitochondrial genome oligo pool, and no-primary-probe control. ( F ) Log 2 fold change of proteins compared to no-primary-probe control, grouped by HPA subcellular location. Significance calculated based on Welch’s t -test for pairwise comparisons (****p-value <0.0001). Log 2 fold change of proteins compared to mitochondrial probe enriched proteins for the RNA Polymerases ( G ), mtDNA nucleoid packaging proteins ( H ), Shelterin ( I ), and CENP-A nucleosomal complexes ( J ). Significance calculated based on Welch’s t -test for pairwise comparisons (p-value: *<0.05, **<0.01, ***<0.001, ****<0.0001).
Am9914g Dna Clean Concentrator 100 Zymo Research, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+clean+concentrator+100/DNA+Clean+%26+Concentrator-100/pm37235474-164-37-42
Average 99 stars, based on 1 article reviews
am9914g dna clean concentrator 100 zymo research - by Bioz Stars, 2026-11
99/100 stars
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( A ) Workflow of DNA O-MAP integrated with sample multiplexing quantitative proteomics. ( B ) Schematic of the three DNA loci examined in the TMT16plex experiment: pericentromeric alpha satellites, telomeres, and mitochondrial genomes. ( C ) Co-localization of DNA fluorescent in situ hybridization (FISH) and the streptavidin staining of the proteins biotinylated by DNA O-MAP targeting the pericentromeric alpha satellites, telomeres, and mitochondrial genomes. Scale bar: 5 µm. ( D ) Principal component analysis of scaled intensities of proteins enriched by the pan-alpha probe, telomere probe, mitochondrial genome oligo pool, and no-primary-probe control. ( E ) Unsupervised hierarchical clustering of scaled intensities of proteins enriched by the pan-alpha probe, telomere probe, mitochondrial genome oligo pool, and no-primary-probe control. ( F ) Log 2 fold change of proteins compared to no-primary-probe control, grouped by HPA subcellular location. Significance calculated based on Welch’s t -test for pairwise comparisons (****p-value <0.0001). Log 2 fold change of proteins compared to mitochondrial probe enriched proteins for the RNA Polymerases ( G ), mtDNA nucleoid packaging proteins ( H ), Shelterin ( I ), and CENP-A nucleosomal complexes ( J ). Significance calculated based on Welch’s t -test for pairwise comparisons (p-value: *<0.05, **<0.01, ***<0.001, ****<0.0001).

Journal: eLife

Article Title: DNA O-MAP uncovers the molecular neighborhoods associated with specific genomic loci

doi: 10.7554/eLife.102489

Figure Lengend Snippet: ( A ) Workflow of DNA O-MAP integrated with sample multiplexing quantitative proteomics. ( B ) Schematic of the three DNA loci examined in the TMT16plex experiment: pericentromeric alpha satellites, telomeres, and mitochondrial genomes. ( C ) Co-localization of DNA fluorescent in situ hybridization (FISH) and the streptavidin staining of the proteins biotinylated by DNA O-MAP targeting the pericentromeric alpha satellites, telomeres, and mitochondrial genomes. Scale bar: 5 µm. ( D ) Principal component analysis of scaled intensities of proteins enriched by the pan-alpha probe, telomere probe, mitochondrial genome oligo pool, and no-primary-probe control. ( E ) Unsupervised hierarchical clustering of scaled intensities of proteins enriched by the pan-alpha probe, telomere probe, mitochondrial genome oligo pool, and no-primary-probe control. ( F ) Log 2 fold change of proteins compared to no-primary-probe control, grouped by HPA subcellular location. Significance calculated based on Welch’s t -test for pairwise comparisons (****p-value <0.0001). Log 2 fold change of proteins compared to mitochondrial probe enriched proteins for the RNA Polymerases ( G ), mtDNA nucleoid packaging proteins ( H ), Shelterin ( I ), and CENP-A nucleosomal complexes ( J ). Significance calculated based on Welch’s t -test for pairwise comparisons (p-value: *<0.05, **<0.01, ***<0.001, ****<0.0001).

Article Snippet: Final ssDNA probe was purified using a Zymo DNA Clean and Concentrator-100 (DCC-100) kit according to the manufacturer’s protocol for oligo purification.

Techniques: Multiplexing, Quantitative Proteomics, In Situ Hybridization, Staining, Control